Responses of Mycobacterium tuberculosis to growth in the mouse lung

被引：70

作者：

Dubnau, E

Chan, J

Mohan, VP

Smith, I

机构：

[1] Publ Hlth Res Inst, TB Ctr, Newark, NJ 07103 USA

[2] Montefiore Med Ctr, Bronx, NY 10467 USA

来源：

INFECTION AND IMMUNITY | 2005年 / 73卷 / 06期

关键词：

D O I：

10.1128/IAI.73.6.3754-3757.2005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Using a promoter trap, we have identified 56 Mycobacterium tuberculosis genes preferentially expressed in the mouse lung. Quantitative real-time PCR showed that RNA levels of several genes were higher from bacteria growing in mouse lungs than from broth cultures. These results support the current hypothesis that Mycobacterium tuberculosis utilizes fatty acids as a carbon source in the mouse lung.

引用

收藏

页码：3754 / 3757

页数：4

相关论文

共 20 条

[1] INHA, A GENE ENCODING A TARGET FOR ISONIAZID AND ETHIONAMIDE IN MYCOBACTERIUM-TUBERCULOSIS [J].

BANERJEE, A ;

DUBNAU, E ;

QUEMARD, A ;

BALASUBRAMANIAN, V ;

UM, KS ;

WILSON, T ;

COLLINS, D ;

DELISLE, G ;

JACOBS, WR .

SCIENCE, 1994, 263 (5144) :227-230

[2] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence [J].

Cole, ST ;

Brosch, R ;

Parkhill, J ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Gordon, SV ;

Eiglmeier, K ;

Gas, S ;

Barry, CE ;

Tekaia, F ;

Badcock, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Connor, R ;

Davies, R ;

Devlin, K ;

Feltwell, T ;

Gentles, S ;

Hamlin, N ;

Holroyd, S ;

Hornby, T ;

Jagels, K ;

Krogh, A ;

McLean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Osborne, J ;

Quail, MA ;

Rajandream, MA ;

Rogers, J ;

Rutter, S ;

Seeger, K ;

Skelton, J ;

Squares, R ;

Squares, S ;

Sulston, JE ;

Taylor, K ;

Whitehead, S ;

Barrell, BG .

NATURE, 1998, 393 (6685) :537-+

[3] MUTATION OF THE PRINCIPAL SIGMA-FACTOR CAUSES LOSS OF VIRULENCE IN A STRAIN OF THE MYCOBACTERIUM-TUBERCULOSIS COMPLEX [J].

COLLINS, DM ;

KAWAKAMI, RP ;

DELISLE, GW ;

PASCOPELLA, L ;

BLOOM, BR ;

JACOBS, WR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) :8036-8040

[4] Mycobacterium tuberculosis genes induced during infection of human macrophages [J].

Dubnau, E ;

Fontán, P ;

Manganelli, R ;

Soares-Appel, S ;

Smith, I .

INFECTION AND IMMUNITY, 2002, 70 (06) :2787-2795

[5] The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages [J].

Gold, B ;

Rodriguez, GM ;

Marras, SAE ;

Pentecost, M ;

Smith, I .

MOLECULAR MICROBIOLOGY, 2001, 42 (03) :851-865

[6]

Kamath AT, 1999, INFECT IMMUN, V67, P1702

[7] Immunogenicity of DNA vaccines expressing tuberculosis proteins fused to tissue plasminogen activator signal sequences [J].

Li, ZM ;

Howard, A ;

Kelley, C ;

Delogu, G ;

Collins, F ;

Morris, S .

INFECTION AND IMMUNITY, 1999, 67 (09) :4780-4786

[8] Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M-bovis [J].

Mahairas, GG ;

Sabo, PJ ;

Hickey, MJ ;

Singh, DC ;

Stover, CK .

JOURNAL OF BACTERIOLOGY, 1996, 178 (05) :1274-1282

[9] Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase [J].

McKinney, JD ;

zu Bentrup, KH ;

Muñoz-Elias, EJ ;

Miczak, A ;

Chen, B ;

Chan, WT ;

Swenson, D ;

Sacchettini, JC ;

Jacobs, WR ;

Russell, DG .

NATURE, 2000, 406 (6797) :735-738

[10] Identification and mutagenesis by allelic exchange of choE, encoding a cholesterol oxidase from the intracellular pathogen Rhodococcus equi [J].

Navas, J ;

González-Zorn, B ;

Ladrón, N ;

Garrido, P ;

Vázquez-Boland, JA .

JOURNAL OF BACTERIOLOGY, 2001, 183 (16) :4796-4805