Structural and mechanical properties of UV-photo-cross-linked poly(N-vinyl-2-pyrrolidone) hydrogels

被引:53
作者
D'Erricot, Gerardino [3 ,4 ]
De Lellis, Marco [4 ]
Mangiapia, Gaetano [3 ,4 ]
Tedeschi, Annarnaria [3 ,4 ]
Ortona, Ornella [3 ,4 ]
Fusco, Sabato [1 ,2 ]
Borzacchiello, Assunta [1 ,2 ]
AmbrosiO, Luigi [1 ,2 ]
机构
[1] Univ Naples Federico II, Ctr Ric Interdipartimentale Biomat, Ist Mat Compositi Biomed CNR, I-80125 Naples, Italy
[2] Univ Naples Federico II, Ctr Ric Interdipartimentale Biomat, CRIB, I-80125 Naples, Italy
[3] Univ Naples Federico II, CSGI, I-80125 Naples, Italy
[4] Univ Naples Federico II, Dipartmento Chim, I-80126 Naples, Italy
关键词
D O I
10.1021/bm7008137
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Biocompatible poly(N-vinyl-2-pyrrolidone) (PVP) hydrogels have been produced by UV irradiation of aqueous polymer mixtures, using a high-pressure mercury lamp. The resulting materials have been characterized by a combination of experimental techniques, including rheology, small-angle neutron scattering (SANS), electron paramagnetic resonance (EPR), and pulsed gradient spin-echo nuclear magnetic resonance (PGSE-NMR), to put in evidence the relationship between the microstructural properties and the macrofunctional behavior of the gels. Viscoelastic measurements showed that UV photo-cross-linked PVP hydrogels present a strong gel mechanical behavior and viscoelastic moduli values similar to those of biological gels. The average distance between the cross-linking points of the polymer network was estimated from the hydrogels elastic modulus. However, SANS measurements showed that the network microstructure is highly inhomogeneous, presenting polymer-rich regions more densely cross-linked, surrounded by a water-rich environment. EPR and PGSE-NMR data further support the existence of these water-rich domains. Inclusion of a third component, such as glycerol, in the PVP aqueous mixture to be irradiated has been also investigated. A small amount of glycerol (<3% w/w) can be added keeping satisfactory properties of the hydrogel, while higher amounts significantly affect the cross-linking process.
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页码:231 / 240
页数:10
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