Structures of complete RNA polymerase II and its subcomplex, Rpb4/7

被引：186

作者：

Armache, KJ ^{[1
]}

Mitterweger, S ^{[1
]}

Meinhart, A ^{[1
]}

Cramer, P ^{[1
]}

机构：

[1] Univ Munich, Gene Ctr, LMU, Dept Chem & Biochem, D-81377 Munich, Germany

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2005年 / 280卷 / 08期

关键词：

D O I：

10.1074/jbc.M413038200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We determined the x-ray structure of the RNA polymerase (Pol) II subcomplex Rpb4/7 at 2.3 Angstrom resolution, combined it with a previous structure of the 10-subunit polymerase core, and refined an atomic model of the complete 12-subunit Pol II at 3.8-Angstrom resolution. Comparison of the complete Pol II structure with structures of the Pol II core and free Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of an a-helix in the linker region of the largest Pol II subunit and with folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7 interface explain facilitated Rpb4/7 dissociation in a temperature-sensitive Pol 11 mutant and specific assembly of Pol I with its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves as the new reference structure for analysis of the transcription mechanism and enables structure solution of complexes of the complete enzyme with additional factors and nucleic acids by molecular replacement.

引用

页码：7131 / 7134

页数：4

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