A semisynthetic fetal calf serum-free liquid medium for in vitro cultivation of Leishmania promastigotes

被引：16

作者：

Ali, SA ^{[1
]}

Iqbal, J

Ahmad, B

Masoom, M

机构：

[1] Univ Balochistan, Inst Biochem, Quetta, Pakistan

[2] Univ Balochistan, Dept Pharm, Quetta, Pakistan

[3] Punjab Univ, Fac Pharm, Lahore, Pakistan

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1998年 / 59卷 / 01期

关键词：

D O I：

10.4269/ajtmh.1998.59.163

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A semisynthetic, autoclavable, liquid medium that does not require inclusion of fetal calf serum (FCS) for the successful cultivation of Leishmania promastigotes in vitro is described. The parasites were grown in commercially available medium 199 supplemented with 10% FCS and 2% mammalian urine and the growth was compared with the same medium without FCS. The growth of the parasites in the semidefined medium without FCS was found to be as good as or slightly better that with medium 199 supplemented with FCS.

引用

页码：163 / 165

页数：3

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