Computer aided data acquisition and analysis of acetlycholinesterase velocity sedimentation profiles

被引：5

作者：

Ribaric, S ^{[1
]}

Peterec, D ^{[1
]}

Sketelj, J ^{[1
]}

机构：

[1] UNIV LJUBLJANA,FAC MED,INST PHYSIOL,LJUBLJANA 61000,SLOVENIA

来源：

COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE | 1996年 / 49卷 / 02期

关键词：

computer-aided data acquisition; acetylcholinesterase (AChE); sedimentation profile;

D O I：

10.1016/0169-2607(96)01719-1

中图分类号：

TP39 [计算机的应用];

学科分类号：

081203 ; 0835 ;

摘要：

Acetylcholinesterase (AChE) is an enzyme that appears in several molecular forms. Individual AChE molecular forms can be isolated and identified on the basis of their velocity sedimentation profiles. We have improved an existing method for analyzing AChE sedimentation profiles with a computer aided data acquisition and analysis system. The software facilitates measurements of AChE activity of individual molecular forms and comparison among AChE velocity sedimentation profiles. In practice, our program increased precision and reduced the time of AChE velocity sedimentation profile analysis. The software can also be applied to velocity sedimentation analysis of other proteins. We plan to upgrade the program by making it compatible with Microsoft Windows.

引用

页码：149 / 156

页数：8

共 10 条

[1] CERNEFINDERLE N, 1995, J NEUROSCI RES, V41, P745
[2] SATELLITE CELLS IN SLOW AND FAST RAT MUSCLES DIFFER IN RESPECT TO ACETYLCHOLINESTERASE REGULATION MECHANISMS THEY CONVEY TO THEIR DESCENDANT MYOFIBERS DURING REGENERATION
DOLENC, I
CRNEFINDERLE, N
SKETELJ, J
ERZEN, I
[J]. JOURNAL OF NEUROSCIENCE RESEARCH, 1994, 37 (02) : 236 - 246
[3] POLYMORPHISM OF ACETYLCHOLINESTERASE AND IDENTIFICATION OF NEW MOLECULAR-FORMS AFTER SEDIMENTATION ANALYSIS
DREYFUS, PA
FRIBOULET, A
TRAN, LH
RIEGER, F
[J]. BIOLOGY OF THE CELL, 1984, 51 (01) : 35 - 41
[4] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY
ELLMAN, GL
COURTNEY, KD
ANDRES, V
FEATHERSTONE, RM
[J]. BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) : 88 - &
[5] MARTIN RG, 1961, J BIOL CHEM, V236, P1372
[6] THE POLYMORPHISM OF CHOLINESTERASES AND ITS PHYSIOLOGICAL SIGNIFICANCE
MASSOULIE, J
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1980, 5 (06) : 160 - 164
[7] ACETYLCHOLINESTERASE IN ELECTRIC ORGANS OF FISH (TORPEDO AND GYMNOTID) AND MEMBRANE SYSTEMS
MASSOULIE, J
RIEGER, F
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 11 (03): : 441 - +
[8] RIBARIC S, 1994, RESTOR NEUROL NEUROS, V7, P89, DOI 10.3233/RNN-1994-7204
[9] MOLECULAR-FORMS AND LOCALIZATION OF ACETYLCHOLINESTERASE AND NONSPECIFIC CHOLINESTERASE IN REGENERATING SKELETAL-MUSCLES
SKETELJ, J
CRNE, N
BRZIN, M
[J]. NEUROCHEMICAL RESEARCH, 1987, 12 (02) : 159 - 165
[10] SKETELJ J, 1993, J NEUROCHEM, V61, P501

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