The production and evaluation of antibodies for enzyme immunoassay of AZTTP

被引：3

作者：

Akeb, F

Creminon, C

Grassi, J

Guedj, R

Duval, D

机构：

[1] Univ Nice, CNRS, Chim Bioorgan Lab, ESA 6001,Fac Sci, F-06108 Nice 2, France

[2] CEA Saclay, Serv Pharmacol & Immunol, Direct Sci Vivant, F-91191 Gif Sur Yvette, France

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 03期

关键词：

D O I：

10.1081/NCN-100002084

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the development of the first enzyme immunoassay for quantifying AZTTP that does not use of radioactive labeling. Anti-AZTTP antibodies were raised in rabbits by immunizing with an AZTTP-kelhoyle limpet hemocyanin (KLH) conjugate. Competitive immunoassays indicated a nanomolar sensitivity to AZTTP. One of the antisera produced was specific for AZTTP.

引用

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页码：243 / 250

页数：8

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