Anti-inflammatory deficiencies in neutrophilic asthma: reduced galectin-3 and IL-1RA/IL-1β

被引：76

作者：

Gao, Peng ^{[1
,2
,3
]}

Gibson, Peter G. ^{[2
,3
,4
]}

Baines, Katherine J. ^{[2
,3
]}

Yang, Ian A. ^{[5
,6
]}

Upham, John W. ^{[5
,7
]}

Reynolds, Paul N. ^{[8
,9
]}

Hodge, Sandra ^{[8
,9
]}

James, Alan L. ^{[10
,11
]}

Jenkins, Christine ^{[12
]}

Peters, Matthew J. ^{[13
,14
]}

Zhang, Jie ^{[1
]}

Simpson, Jodie L. ^{[2
,3
]}

机构：

[1] Jilin Univ, Affiliated Hosp 2, Dept Resp Med, Changchun 130023, Jilin, Peoples R China

[2] Hunter Med Res Inst, Dept Resp & Sleep Med, New Lambton Heights, NSW, Australia

[3] Univ Newcastle, Prior Res Ctr Asthma & Resp Dis, Callaghan, NSW 2308, Australia

[4] Woolcock Inst Med Res, Glebe, NSW, Australia

[5] Univ Queensland, Sch Med, Brisbane, Qld, Australia

[6] Prince Charles Hosp, Dept Thorac Med, Brisbane, Qld 4032, Australia

[7] Princess Alexandra Hosp, Dept Resp Med, Brisbane, Qld 4102, Australia

[8] Royal Adelaide Hosp, Dept Thorac Med, Adelaide, SA 5000, Australia

[9] Hanson Inst, Lung Res Lab, Adelaide, SA, Australia

[10] Sir Charles Gairdner Hosp, Dept Pulm Physiol & Sleep Med, Perth, WA, Australia

[11] Univ Western Australia, Sch Med & Pharmacol, Perth, WA 6009, Australia

[12] George Inst Global Hlth, Sydney, NSW, Australia

[13] Macquarie Univ, Australian Sch Adv Med, Sydney, NSW 2109, Australia

[14] Concord Gen Hosp, Dept Thorac Med, Concord, NSW, Australia

来源：

RESPIRATORY RESEARCH | 2015年 / 16卷

关键词：

Asthma; Galectin-3; Induced sputum; Neutrophil; Macrophage; IL-1; beta; lectin; C-ASSOCIATED PROTEIN; AIRWAY INFLAMMATION; MURINE MODEL; EXPRESSION; ACTIVATION; HYPERRESPONSIVENESS; IDENTIFICATION; GLYCOPROTEIN; CYTOKINES; CELLS;

D O I：

10.1186/s12931-014-0163-5

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

100201 [内科学];

摘要：

Background: Galectin-3 (gal-3), a member of the beta-galactoside-binding animal lectins, is involved in the recruitment, activation and removal of neutrophils. Neutrophilic asthma is characterized by a persistent elevation of airway neutrophils and impaired efferocytosis. We hypothesized that sputum gal-3 would be reduced in neutrophilic asthma and the expression of gal-3 would be associated with other markers of neutrophilic inflammation. Methods: Adults with asthma (n = 80) underwent a sputum induction following clinical assessment and blood collection. Sputum was dispersed for a differential cell count and ELISA assessment of gal-3, gal-3 binding protein (BP), interleukin (IL)-1 beta, IL-1 receptor antagonist (RA), IL-8 and IL-6. Gal-3 and gal-3BP immunoreactivity were assessed in mixed sputum cells. Results: Sputum gal-3 (median, (q1, q3)) was significantly reduced in neutrophilic asthma (183 ng/mL (91,287)) compared with eosinophilic (293 ng/mL (188,471), p = 0.021) and paucigranulocytic asthma (399 ng/mL (213,514), p = 0.004). The gal-3/gal-3BP ratio and IL-1RA/IL-1 beta ratio were significantly reduced, while gal-3BP and IL-1 beta were significantly elevated in neutrophilic asthma compared with eosinophilic and paucigranulocytic asthma. Conclusion: Patients with neutrophilic asthma have impairment in anti-inflammatory ratio of gal-3/gal-3BP and IL-1RA/IL-1 beta which provides a further framework for exploration into pathologic mechanisms of asthma phenotypes.

引用

页数：10

共 44 条

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