DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: A novel adjuvant and delivery system

被引：25

作者：

Carneiro, Catarina ^{[1
]}

Correia, Alexandra ^{[2
]}

Collins, Tony ^{[1
]}

Vilanova, Manuel ^{[2
,3
]}

Pais, Celia ^{[1
]}

Gomes, Andreia C. ^{[1
,5
]}

Oliveira, M. Elisabete C. D. Real ^{[4
,5
]}

Sampaio, Paula ^{[1
]}

机构：

[1] Univ Minho, Ctr Mol & Environm Biol CBMA, Dept Biol, P-4710057 Braga, Portugal

[2] Inst Biol Mol & Celular, Oporto, Portugal

[3] Univ Porto, Inst Ciencias Biomed Abel Salazar, P-4100 Oporto, Portugal

[4] Univ Minho, Ctr Phys CFUM, P-4710057 Braga, Portugal

[5] NanoDelivery I&D Biotechnol, Dept Biol, Braga, Portugal

来源：

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS | 2015年 / 89卷

关键词：

DODAB:MO liposomes; Nanoparticles; Adjuvant activity; Antigen delivery systems; Candida albicans proteins; Liposome endocytosis; Humoral and cellular immune responses; Antigen protection; VACCINE DELIVERY; DENDRITIC CELLS; MEDIATED-IMMUNITY; CONJUGATE VACCINE; FUNGAL PATHOGENS; IN-VIVO; MACROPHAGES; ANTIBODY; MANNAN; LIPIDS;

D O I：

10.1016/j.ejpb.2014.11.028

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We describe the preparation and characterization of DODAB:MO-based liposomes and demonstrate their adjuvant potential and use in antigen delivery. Liposomes loaded with Candida albicans proteins assembled as stable negatively charged spherical nanoparticles with a mean size of 280 nm. High adsorption efficiency (91.0 +/- 9.0%) is attained with high lipid concentrations. The nanoparticles were non-toxic, avidly taken up by macrophage cells and accumulated in membrane rich regions with an internalization time of 20 min. Immunized mice displayed strong humoral and cell-mediated immune responses, producing antibodies (IgGs) against specific cell wall proteins, Cht3p and Xog1p. DODAB:MO-based liposomes loaded with C. albicans proteins have an excellent immunogenic potential and can be explored for the development of an immunoprotective strategy against Candida infections. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：190 / 200

页数：11

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