Identification of DNA binding-site preferences for nuclear factor I-A

被引：26

作者：

Osada, S ^{[1
]}

Daimon, S ^{[1
]}

Nishihara, T ^{[1
]}

Imagawa, M ^{[1
]}

机构：

[1] OSAKA UNIV,FAC PHARMACEUT SCI,DEPT ENVIRONM BIOCHEM,SUITA,OSAKA 565,JAPAN

来源：

FEBS LETTERS | 1996年 / 390卷 / 01期

关键词：

nuclear factor I; transcription factor; DNA binding site; consensus sequence;

D O I：

10.1016/0014-5793(96)00622-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear factor I (NFI) proteins constitute a large family of DNA binding proteins, These proteins promote the initiation of adenovirus replication and regulate the transcription of viral and cellular genes, The binding sites for NFI have been reported in a wide variety of promoters, and they exhibit flexibility in their sequences, To clarify fhe DKA binding site of NFI-A, one of the NFI proteins, rue performed a polymerase chain reaction-mediated random site selection, and determined the optimal sequence as 5'-TTGGCANNNN(G/T)CCA(G/A)-3'.

引用

页码：44 / 46

页数：3

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