Gene discovery through expressed sequence tag sequencing in Trypanosoma cruzi

被引:48
作者
Verdun, RE
Di Paolo, N
Urmenyi, TP
Rondinelli, E
Frasch, ACC
Sanchez, DO
机构
[1] Univ Nacl Gen San Martin, INTI Ed 24, Inst Invest Biotecnol, RA-1650 San Martin, Buenos Aires, Argentina
[2] Fed Univ Rio De Janeiro, Inst Biofis Carlos Chagas Filho, Rio De Janeiro, Brazil
关键词
D O I
10.1128/IAI.66.11.5393-5398.1998
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Analysis of expressed sequence tags (ESTs) constitutes a useful approach for gene identification that, in the case of human pathogens, might result in the identification of new targets for chemotherapy and vaccine development. As part of the Trypanosoma cruzi genome project, we have partially sequenced the 5' ends of 1,949 clones to generate ESTs, The clones were randomly selected from a normalized CL Brener epimastigote cDNA library. A total of 14.6% of the clones were homologous to previously identified T. cruzi genes, while 18.4% had significant matches to genes from other organisms in the database. A total of 67% of the ESTs had no matches in the database, and thus, some of them might be T. cruzi-specific genes. Functional groups of those sequences with matches in the database were constructed according to their putative biological functions, The two largest categories were protein synthesis (23.3%) and cell surface molecules (10.8%). The information reported in this paper should be useful for researchers in the field to analyze genes and proteins of their own interest.
引用
收藏
页码:5393 / 5398
页数:6
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