Binding of the initiation factor σ70 to core RNA polymerase is a multistep process

被引：59

作者：

Gruber, TM

Markov, D

Sharp, MM

Young, BA

Lu, CZ

Zhong, HJ

Artsimovitch, I

Geszvain, KM

Arthur, TM

Burgess, RR

Landick, R

Severinov, K

Gross, CA ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Stomatol, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

[4] Rutgers State Univ, Dept Genet, Piscataway, NJ 08854 USA

[5] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

[6] Univ Wisconsin, Dept Oncol, Madison, WI 53706 USA

来源：

MOLECULAR CELL | 2001年 / 8卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(01)00292-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of RNA polymerase and its initiation factors is central to the process of transcription initiation. To dissect the role of this interface, we undertook the identification of the contact sites between RNA polymerase and sigma (70), the Escherichia coli initiation factor. We identified nine mutationally verified interaction sites between sigma (70) and specific domains of RNA polymerase and provide evidence that sigma (70) and RNA polymerase interact in at least a two-step process. We propose that a cycle of changes in the interface of sigma (70) with core RNA polymerase is associated with progression through the process of transcription initiation.

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页码：21 / 31

页数：11

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