Identification of progesterone-dependent messenger ribonucleic acid regulatory patterns in the rhesus monkey endometrium by differential-display reverse transcription polymerase chain reaction

被引:10
作者
Ace, CI
Okulicz, WC
机构
[1] Univ Massachusetts, Sch Med, Dept OB GYN, Worcester, MA 01655 USA
[2] Univ Massachusetts, Sch Med, Dept Physiol, Worcester, MA 01655 USA
关键词
D O I
10.1095/biolreprod60.4.1029
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We used differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) to identify different patterns of progesterone (P-4)-dependent gene regulation in rhesus monkey endometria. Complementary DNA populations representing the proliferative phase (estrogen dominant, EcDNA) and an inadequate secretory phase (low level of P-4, IcDNA) were compared with a cDNA population representing an adequate secretory phase (normal level of P-4, PcDNA). We were able to distinguish four different levels of mRNA regulation: 1) up-regulation by P-4 during an adequate secretory phase, 2) autologous down-regulation (IcDNA versus PcDNA), 3) lower abundance in IcDNA compared to PcDNA, and 4) P-4-dependent inhibition of EcDNA gene expression. We isolated and sequenced 16 fragments representing these different levels of P-4 regulation. The sequence of thr ee fragments that were autologously down-regulated (11, 12, 14) matched previously entered GenBank mRNAs: II encodes serine/threonine protein phosphatase A; 12 encodes oxobutanoate dehydrogenase E1b-beta; and 14 encodes line-1 reverse transcriptase homologue. Six other fragments exhibited homology to uncharacterized expressed sequence tags, sequence site tag, and cosmid clones. The remaining seven fragments exhibited no significant homology to GenBank entries at this time. The various patterns of P-4-dependent gene regulation identified in the present study are likely to play roles in the temporal orchestration of events that lead to proper maturation of the endometrium.
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页码:1029 / 1035
页数:7
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