Biochemical characterization of osteo-testicular protein tyrosine phosphatase and its functional significance in rat primary osteoblasts

被引：22

作者：

Chengalvala, MV ^{[1
]}

Bapat, AR ^{[1
]}

Hurlburt, WW ^{[1
]}

Kostek, B ^{[1
]}

Gonder, DS ^{[1
]}

Mastroeni, RA ^{[1
]}

Frail, DE ^{[1
]}

机构：

[1] Wyeth Ayerst Res, Womens Hlth Res Inst, Div Mol Biol, Radnor, PA 19087 USA

来源：

BIOCHEMISTRY | 2001年 / 40卷 / 03期

关键词：

D O I：

10.1021/bi0019996

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat osteo-testicular protein tyrosine phosphatase (OST-PTP), expressed in osteoblasts and testis, is a receptor-like transmembrane protein with two tandemly repeated phosphatase domains in the cytoplasmic region. In this report, we show that the first domain (CD1) is enzymatically active and appears to be influenced by the catalytically inactive second domain (CD2). The activity of CD1 is specific to phosphorylated tyrosine, Full-length OST-PTP protein expressed in COS cells has a molecular mass of approximately 185 kDa, and immunoprecipitates of this protein using OST-PTP-specific antisera show strong tyrosine phosphatase activity. Expression of OST-PTP mRNA in primary rat calvarial osteoblasts is temporally regulated, and peak expression is found at approximately day 15, which correlated well with the appearance of OST-PTP protein and its associated tyrosine phosphatase activity. Treatment of osteoblasts in culture with antisense oligonucleotides directed against the 5' untranslated region of OST-PTP results in abrogation of differentiation, confirming the functional importance of OST-PTP expression in osteoblast development.

引用

页码：814 / 821

页数：8

共 28 条

[1] FACTORS THAT PROMOTE PROGRESSIVE DEVELOPMENT OF THE OSTEOBLAST PHENOTYPE IN CULTURED FETAL-RAT CALVARIA CELLS
ARONOW, MA
GERSTENFELD, LC
OWEN, TA
TASSINARI, MS
STEIN, GS
LIAN, JB
[J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 143 (02) : 213 - 221
[2] BRADYKALNAY SM, 1994, J BIOL CHEM, V269, P28472
[3] BRADYKALNAY SM, 1993, ADV PROTEIN PHOSPHAT, V8, P241
[4] CHARBONNEAU H, 1992, ANNU REV CELL BIOL, V8, P463, DOI 10.1146/annurev.cellbio.8.1.463
[5] Clark G., 1981, STAINING PROCEDURES, P187
[6] Dixon JE, 1996, RECENT PROG HORM RES, V51, P405
[7] PROTEIN TYROSINE PHOSPHATASES - A DIVERSE FAMILY OF INTRACELLULAR AND TRANSMEMBRANE ENZYMES
FISCHER, EH
CHARBONNEAU, H
TONKS, NK
[J]. SCIENCE, 1991, 253 (5018) : 401 - 406
[8] Comparisons of catalytic selectivity of cytochrome P450 subfamily enzymes from different species
Guengerich, FP
[J]. CHEMICO-BIOLOGICAL INTERACTIONS, 1997, 106 (03) : 161 - 182
[9] GUNDBERG CM, 1984, METHOD ENZYMOL, V173, P534
[10] HUIJSDUIJNEN RH, 1998, GENE, V225, P1

← 1 2 3 →