High resolution free chromatin/DNA fiber fluorescent in situ hybridization

被引：29

作者：

Heng, HHQ

Tsui, LC

机构：

[1] Hosp Sick Children, Dept Genet, Toronto, ON M5G 1X8, Canada

[2] York Univ, Dept Biol, Toronto, ON M3J 1P3, Canada

[3] SeeDNA Biotech Inc, Toronto, ON, Canada

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 806卷 / 01期

关键词：

fluorescent in situ hybridization (FISH); physical mapping; chromatin fibers; DNA fibers; chromosomes; gene mapping; genome analysis; detection; reviews;

D O I：

10.1016/S0021-9673(98)00150-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High resolution chromatin/DNA fiber fluorescent in situ hybridisation (FISH) is a powerful system for physical mapping and genome research. With direct visualisation of molecular probes along released chromatin or DNA fiber, fiber FISH has become the method of choice to order genes or DNA markers within chromosomal regions of interest. Combined with DNA-protein in situ codetection fiber FISH shall play a more important role for analysis of genome function. In this paper the concept and technical developments of fiber FISH are reviewed with the emphasis of comparison on the various protocols. Future challenges are also discussed along with the highlights of the successful applications achieved by fiber FISH methodology. (C) 1998 Elsevier Science B.V.

引用

页码：219 / 229

页数：11

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