Exon junction complexes mediate the enhancing effect of splicing on mRNA expression

被引:175
作者
Wiegand, HL
Lu, SH
Cullen, BR [1 ]
机构
[1] Duke Univ, Med Ctr, Howard Hughes Med Inst, Durham, NC 27710 USA
[2] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA
关键词
D O I
10.1073/pnas.1934877100
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Intron-containing genes are generally expressed more effectively in Niman cells than are intronless versions of the same gene. We have asked whether this effect is due directly to splicing or instead reflects the action of components of the exon junction complex (EJC), that is assembled at splice junctions after splicing is completed. Here, we show that intron removal does not enhance gene expression if EJC formation is blocked. Conversely, RNA tethering of the EJC components SRm160 or RNPS1 boosts the expression of intronless mRNAs but not of spliced mRNAs. Splicing and RNPS1 tethoring are shown to enhance the same steps in mRNA biogenesis and function, including mRNA 3' end processing and translation. Together, these data argue that the EJC is primarily responsible for the positive effect of splicing on gene expression.
引用
收藏
页码:11327 / 11332
页数:6
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