A novel method for producing electron transparent films of interfaces between cells and biomaterials

被引：11

作者：

Engqvist, Hakan ^{[1
]}

Svahn, Fredrik

Jarmar, Tobias ^{[1
]}

Detsch, Rainer ^{[2
]}

Mayr, Helmar ^{[3
]}

Thomsen, Peter ^{[4
]}

Ziegler, Guenter ^{[2
,3
]}

机构：

[1] Doxa AB, S-75451 Uppsala, Sweden

[2] BioCer Entwicklungs GmbH, D-95447 Bayreuth, Germany

[3] Friedrich Baur Res Inst Biomat, D-95440 Bayreuth, Germany

[4] Gothenburg Univ, Sahlgrenska Acad, Dept Biomat, S-40530 Gothenburg, Sweden

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2008年 / 19卷 / 01期

关键词：

D O I：

10.1007/s10856-006-0042-9

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Transmission electron microscopy (TEM) investigations of intact interfaces of cells and brittle biomaterials have proven difficult using common TEM preparation techniques. This paper describes a technique to fabricate thin sections for TEM investigation of intact interfaces between human monocytes and sintered hydroxylapatite by the use of focused ion beam (FIB) microscopy. The interfaces were examined using energy filtered TEM.

引用

页码：467 / 470

页数：4

共 9 条

[1] Electron energy loss spectroscopy microanalysis and imaging in the transmission electron microscope: example of biological applications [J].