Isolation and characterization of a sialo-glycopeptide from buffalo colostrum

被引：6

作者：

Aparna, HS ^{[1
]}

Salimath, PV ^{[1
]}

机构：

[1] CENT FOOD TECHNOL RES INST, DEPT BIOCHEM & NUTR, MYSORE 570013, KARNATAKA, INDIA

来源：

GLYCOCONJUGATE JOURNAL | 1996年 / 13卷 / 01期

关键词：

buffalo; colostrum; glycopeptide;

D O I：

10.1007/BF01049681

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sialoglycopeptide was isolated from buffalo colostrum in pure form by chromatography on Sephadex G-25 and QAE-Sephadex A-25. This was found to be homogeneous by cellulose acetate membrane electrophoresis and reverse phase HPLC. it consisted of fucose, galactose, mannose, N-acetyl glucosamine and N-acetyl neuraminic acid in the ratio 1:2:3:4:1, and aspartic acid, serine, threonine, proline and glutamic acid were the major amino acids. Glycine was identified as the N-terminal amino acid residue. The structure elucidation of the carbohydrate moiety was carried out by methylation analysis, mass spectrometry, H-1-NMR spectroscopy and the probable structure was revealed to be that of a complex biantennary type.

引用

页码：63 / 67

页数：5

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