The interaction of polysaccharides with a spectroscopic probe: The anion effect on the binding site of heparin

被引：11

作者：

Liu, Q ^{[1
]}

Jiao, QC ^{[1
]}

Liu, ZL ^{[1
]}

Chen, H ^{[1
]}

机构：

[1] Nanjing Univ, Coll Life Sci, Pharmaceut Biotechnol Key Lab, Nanjing 210093, Peoples R China

来源：

SPECTROSCOPY LETTERS | 2001年 / 34卷 / 01期

关键词：

anion; binding site; heparin; azure A;

D O I：

10.1081/SL-100001447

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Heparin (Hep) has a variety of biological activities, most of them due to Hep's high anionic groups. The average binding number (n) is proposed to express the density of anion's group (binding site) on Hep. To gain insight into the binding site of Hep under the interference of anions, we have developed a method that makes use of isosbestic points in the reaction system in which two reagents compete for the same dye ion, and one complex is colorless. It is also necessary to determine the molar absorptivities of the various species concerned.

引用

页码：25 / 34

页数：10

共 20 条

[1] A mathematical model for the description of the coomassie brilliant blue protein assay [J].

Atherton, BA ;

Cunningham, EL ;

Splittgerber, AG .

ANALYTICAL BIOCHEMISTRY, 1996, 233 (02) :160-168

[2]

BAZZONI G, 1993, J LAB CLIN MED, V121, P268

[3] INTERACTION BETWEEN GLYCOSAMINOGLYCANS, PLATELETS, AND LEUKOCYTES [J].

CERLETTI, C ;

RAJTAR, G ;

MARCHI, E ;

DEGAETANO, G .

SEMINARS IN THROMBOSIS AND HEMOSTASIS, 1994, 20 (03) :245-253

[4]

JAQUES LB, 1990, J LAB CLIN MED, V115, P422

[5] Characterization of the interaction between methylene blue and glycosaminoglycans [J].

Jiao, QC ;

Liu, Q .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 1999, 55 (7-8) :1667-1673

[6] A mathematical model for interaction of spectroscopic probe with polysaccharides - The binding interaction between azure A and heparin [J].

Jiao, QC ;

Liu, Q .

SPECTROSCOPY LETTERS, 1998, 31 (07) :1353-1365

[7] Investigation on the binding site in heparin by spectrophotometry [J].

Jiao, QC ;

Liu, Q ;

Sun, C ;

He, H .

TALANTA, 1999, 48 (05) :1095-1101

[8]

Jiao QC, 1998, ANAL LETT, V31, P1311

[9] MORE TO HEPARIN THAN ANTICOAGULATION [J].

LINDAHL, U ;

LIDHOLT, K ;

SPILLMANN, D ;

KJELLEN, L .

THROMBOSIS RESEARCH, 1994, 75 (01) :1-32

[10] Mechanism of methylene blue action and interference in the heparin assay [J].

Liu, Q ;

Jiao, QC .

SPECTROSCOPY LETTERS, 1998, 31 (05) :913-924

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