Patterns that define the four domains conserved in known and novel isoforms of the protein import receptor Tom20

被引:44
作者
Likic, VA
Perry, A
Hulett, J
Derby, M
Traven, A
Waller, RF
Keeling, PJ
Koehler, CM
Curran, SP
Gooley, PR [1 ]
Lithgow, T
机构
[1] Univ Melbourne, Russell Grimwade Sch Biochem & Mol Biol, Melbourne, Vic 3010, Australia
[2] St Vincents Inst Med Res, Fitzroy, Vic 3065, Australia
[3] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada
[4] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
基金
英国医学研究理事会; 澳大利亚研究理事会;
关键词
mitochondria; protein import; protein targeting sequences; import receptors; hidden Markov models;
D O I
10.1016/j.jmb.2004.12.057
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tom20 is the master receptor for protein import into mitochondria. Analysis of motifs present in Tom20 sequences from fungi and animals found several highly conserved regions, including features of the transmembrane segment, the ligand-binding domain and functionally important flexible segments at the N terminus and the C terminus of the protein. Hidden Markov model searches of genome sequence data revealed novel isoforms of Tom20 in vertebrate and invertebrate animals. A three-dimensional comparative model of the novel type I Tom20, based on the structurally characterized type 11 isoform, shows important differences in the amino acid residues lining the ligand-binding groove, where the type I protein from animals is more similar to the fungal form of Tom20. Given that the two receptor types from mouse interact with the same set of precursor protein substrates, comparative analysis of the substrate-binding site provides unique insight into the mechanism of substrate recognition. No Tom20-related protein was found in genome sequence data from plants or protozoans, suggesting the receptor Tom20 evolved after the split of animals and fungi from the main lineage of eukaryotes. (C) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:81 / 93
页数:13
相关论文
共 74 条
  • [1] Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20
    Abe, Y
    Shodai, T
    Muto, T
    Mihara, K
    Torii, H
    Nishikawa, S
    Endo, T
    Kohda, D
    [J]. CELL, 2000, 100 (05) : 551 - 560
  • [2] The TOM core complex: The general protein import pore of the outer membrane of mitochondria
    Ahting, U
    Thun, C
    Hegerl, R
    Typke, D
    Nargang, FE
    Neupert, W
    Nussberger, S
    [J]. JOURNAL OF CELL BIOLOGY, 1999, 147 (05) : 959 - 968
  • [3] Analysis of cell-type-specific gene expression during mouse spermatogenesis
    Almstrup, K
    Nielsen, JE
    Hansen, MA
    Tanaka, M
    Skakkebæk, NE
    Leffers, H
    [J]. BIOLOGY OF REPRODUCTION, 2004, 70 (06) : 1751 - 1761
  • [4] On the origin of mitochondria:: a genomics perspective
    Andersson, SGE
    Karlberg, O
    Canbäck, B
    Kurland, CG
    [J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2003, 358 (1429) : 165 - 177
  • [5] Gene discovery using computational and microarray analysis of transcription in the Drosophila melanogaster testis
    Andrews, J
    Bouffard, GG
    Cheadle, C
    Lü, JN
    Becker, KG
    Oliver, B
    [J]. GENOME RESEARCH, 2000, 10 (12) : 2030 - 2043
  • [6] Bailey T., 1994, P 2 INT C INT SYST M, P28
  • [7] The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom
    Bauer, MF
    Rothbauer, U
    Mühlenbein, N
    Smith, RJH
    Gerbitz, KD
    Neupert, W
    Brunner, M
    Hofmann, S
    [J]. FEBS LETTERS, 1999, 464 (1-2) : 41 - 47
  • [8] DEVELOPMENT OF HYDROPHOBICITY PARAMETERS TO ANALYZE PROTEINS WHICH BEAR POSTTRANSLATIONAL OR COTRANSLATIONAL MODIFICATIONS
    BLACK, SD
    MOULD, DR
    [J]. ANALYTICAL BIOCHEMISTRY, 1991, 193 (01) : 72 - 82
  • [9] Acidic receptor domains on both sides of the outer membrane mediate translocation of precursor proteins into yeast mitochondria
    Bolliger, L
    Junne, T
    Schatz, G
    Lithgow, T
    [J]. EMBO JOURNAL, 1995, 14 (24) : 6318 - 6326
  • [10] BRENNER S, 1974, GENETICS, V77, P71