A simple route to incorporate redox mediator into carbon nanotubes/Nafion composite film and its application to determine NADH at low potential

被引:43
作者
Huang, Minghua
Jiang, Heqing
Zhai, Junfeng
Liu, Baifeng
Dong, Shaojun
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 10039, Peoples R China
基金
中国国家自然科学基金;
关键词
redox mediator; carbon nanotube; nation; NADH oxidation; low potential determination;
D O I
10.1016/j.talanta.2007.05.042
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Through a new and simple ion-exchange route, two-electron redox mediator thionine has been deliberately incorporated into the carbon nanotubes (CNTs)/Nafion composite film due to the fact that there is strong interaction between any of two among the three materials (ion-exchange process between thionine and Nafion, strong adsorption of thionine by CNTs, and wrapping and solubilizing of CNTs with Nation). The good homogenization of electron conductor CNTs in the integrated films provides the possibility of three-dimensional electron conductive network. The resulting integrated films exhibited high and stable electrocatalytic activity toward NADH oxidation with the significant decrease of high overpotential, which responds more sensitively more than those modified by thioine or CNTs alone. Such high electrocatalytic activity facilitated the low potential determination of NADH (as low as -0.1 V), which eliminated the interferences from other easily oxidizable species. In a word, the immobilization approach is very simple, timesaving and effective, which could be extended to the immobilization of other cationic redox mediators into the CNTs/Nafion composite film. And these features may offer potential promise for the design of amperometric biosensors. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:132 / 139
页数:8
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