The thermal unfolding of native cytochrome c in the transition from solution to gas phase probed by native electron capture dissociation

被引：60

作者：

Breuker, K

McLafferty, FW

机构：

[1] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria

[2] Univ Innsbruck, CMBI, A-6020 Innsbruck, Austria

[3] Cornell Univ, Baker Lab, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 31期

关键词：

hydration; mass spectrometry; native electron capture dissociation; noncovalent interactions; protein structures;

D O I：

10.1002/anie.200500668

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Unfolding upon dehydration:. What happens to the structure of a native protein that is suddenly exposed to vacuum? Native electron capture dissociation shows that dehydration turns the strongest intramolecular interactions in solution into the weakest in the gas phase. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：4911 / 4914

页数：4

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