Detection of pesticide by polymeric enzyme electrodes

被引：25

作者：

Dutta, K. ^{[1
]}

Bhattacharyay, D.

Mukherjee, A. ^{[2
]}

Setford, S. J. ^{[3
]}

Turner, A. P. F. ^{[3
]}

Sarkar, P. ^{[1
]}

机构：

[1] Univ Calcutta, Dept Polymer Sci & Technol, Kolkata 700009, W Bengal, India

[2] Jadavpur Univ, Dept Chem Engn, Kolkata 700032, India

[3] Cranfield Univ, Cranfield Hlth, Silsoe MK45 4DT, Beds, England

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2008年 / 69卷 / 03期

基金：

英国惠康基金;

关键词：

microencapsulation; pesticide; polyacrylamide; potentiometry; screen-printed electrode; biosensor;

D O I：

10.1016/j.ecoenv.2007.01.004

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Screen-printed electrodes (SPEs) containing immobilized acetylcholine esterase (AChE) enzyme were used for the electrochemical determination of organophosphorous (OP) and carbamate pesticides. The extent of AChE deactivation by the pesticide was determined in the presence of acetylcholine (AChCl) substrate. The unique nature of this approach lies in the enzyme immobilization procedure in which AChE was attached to the SPE by in situ bulk polymerization of acrylamide to ensure efficient adherence within the membrane with minimal losses in enzyme activity. Responses were observed for the pesticides Monocrotophos, Malathion, Metasystox and Lannate over the concentration range 0-10 ppb (mu g L-1). (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：556 / 561

页数：6

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