Design and application of fiber-optic evanescent wave biosensor

被引：4

作者：

Huang, HJ ^{[1
]}

Zhai, JH ^{[1
]}

Zhao, YK ^{[1
]}

Yang, RF ^{[1
]}

Ren, BQ ^{[1
]}

Cheng, ZG ^{[1
]}

Du, LL ^{[1
]}

Lu, DW ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China

来源：

PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE | 2003年 / 5254卷

关键词：

biosensor; evanescent wave; optical fiber; nucleic acid;

D O I：

10.1117/12.546217

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fiber-optic biosensor is developed based on the principle of evanescent wave while light propagates in optical fiber. The biosensor uses a red laser diode at 636.85 nm for exciting Cy5 fluorescent dye. Sensitivity limit of 0.01 nmol/l is obtained from the detection of serial Cy5 solutions with various concentrations. In log-to-log plot, excellent linear response characteristic is seen in the Cy5 concentrations ranging from 0.01 nmlo/l to 100 nmol/l. And a good result of signal-to-noise ratio of 4.61 is obtained when the biosensor is used to measure Legionella pneumophila solution of 0.01 mumol/l. All the results are comparable with those that are obtained by a commercial biochip scanner GeneTAC 1000.

引用

页码：316 / 322

页数：7

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