Preparation of an ultrafiltration membrane from the copolymer of acrylonitrile-glycidylmethacrylate utilized for immobilization of glucose oxidase

Ultrafiltration membrane has been prepared from the copolymer of acrylonitrile-glycidylmethacrylate and the porosity of the membrane was studied. The asymmetric structure was proved by scanning electron microscopy. The basic characteristics of the membrane were measured - water permeability, water content, membrane selectivity, etc. The membrane obtained was used as a carrier for immobilization of glucose oxidase. The immobilization was carried out covalently by two methods: direct bonding of the enzyme and indirectly by a spacer (hexamethylenediamine) and cross-linking agent (glutar aldehyde). The amount of bound protein and relative activity of the immobilized glucose oxidase were determined. Temperature optimum, pH optimum and storage stability of the immobilized glucose oxidase were determined. It was proved that glucose oxidase immobilized by the direct method shows better characteristics compared with the indirect method. (C) 1999 Elsevier Science B.V. All rights reserved.