The challenge of merging food safety diagnostic needs with quantitative PCR platforms

被引：38

作者：

Cocolin, Luca ^{[1
]}

Rajkovic, Andreja ^{[2
,3
]}

Rantsiou, Kalliopi ^{[1
]}

Uyttendaele, Mieke ^{[2
]}

机构：

[1] Univ Turin, Fac Agr, DIVAPRA, I-10095 Turin, Italy

[2] Univ Ghent, Fac Biosci Engn, Lab Food Microbiol & Food Preservat, Ghent, Belgium

[3] Univ Belgrade, Fac Agr, Dept Food Safety & Food Qual Management, Belgrade 11001, Serbia

来源：

TRENDS IN FOOD SCIENCE & TECHNOLOGY | 2011年 / 22卷

关键词：

REAL-TIME PCR; VIRAL EXTRACTION METHODS; ESCHERICHIA-COLI O157; TO-EAT FOODS; LISTERIA-MONOCYTOGENES; RAPID DETECTION; ENRICHMENT CONDITIONS; CAMPYLOBACTER-JEJUNI; SALMONELLA-ENTERICA; MEAT-PRODUCTS;

D O I：

10.1016/j.tifs.2011.02.009

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The field of food microbiology and safety has experienced an exciting time in the last 10 years. New methods have been introduced to assess safety of foods by targeting pathogenic microorganisms without the need for cultivation. The most prominent innovation in this field is represented by the Polymerase Chain Reaction. From the 1990s, PCR has seen a tremendous increase in its applications, and from the conventional, qualitative format, nowadays it became quantitative. In this paper we describe its principles and applications in food safety and we critically evaluate its benefits as well as aspects requiring further improvement.

引用

页码：S30 / S38

页数：9

共 62 条

[1] ANALYSIS OF CELL-SIZE AND DNA CONTENT IN EXPONENTIALLY GROWING AND STATIONARY-PHASE BATCH CULTURES OF ESCHERICHIA-COLI [J].