Oligomerization of adenosine A2A and dopamine D2 receptors in living cells

被引：109

作者：

Kamiya, T ^{[1
]}

Saitoh, O ^{[1
]}

Yoshioka, K ^{[1
]}

Nakata, H ^{[1
]}

机构：

[1] Tokyo Metropolitan Inst Neurosci, Dept Mol Cell Signaling, Fuchu, Tokyo 1838526, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 306卷 / 02期

关键词：

oligomerization; adenosine A(2A) receptor; dopamine D-2 receptor; bioluminescence resonance energy transfer; purinergic; striatum;

D O I：

10.1016/S0006-291X(03)00991-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated whether oligomerization of adenosine A(2A) receptor (A(2A)R) and dopamine D-2 receptor (D2R) exists in living cells using modified bioluminescence resonance energy transfer (BRET2) technology. Fusion of these receptors to a donor, Renilla luciferase (Rluc), and to an acceptor, modified green fluorescent protein (GFP(2)), did not affect the ligand binding affinity, subcellular distribution, and coimmunoprecipitation of the receptors. BRET was detected not only between Myc-D2R-Rluc and A(2A)R-GFP(2) but also between HA-tagged A(2A)R-Rluc and A(2A)R-GFP(2). These results indicate A(2A)R, either homomeric or heteromeric with D,R, exists as an oligomer in living cells. (C) 2003 Elsevier Science (USA). All rights reserved.

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页码：544 / 549

页数：6

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