Simple and rapid methods for SEM observation and TEM immunolabeling of rubber particles

被引：13

作者：

Singh, AP ^{[1
]}

Wi, SG

Kang, HS

Chung, GC

Kim, YS

机构：

[1] Chonnam Natl Univ, Agr Plant Stress Ctr, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Dept Forest Prod & Technol, Kwangju 500757, South Korea

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2003年 / 51卷 / 08期

关键词：

rubber particles; air-drying; SEM; TEM immunolabeling;

D O I：

10.1177/002215540305100815

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde-tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving their integrity.

引用

页码：1105 / 1108

页数：4

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