Heart function and molecular biological parameters are comparable in young adult and aged rats after chronic myocardial infarction

Objective: To test the hypothesis that IL-1 beta and IL-6 play a pivotal role after myocardial infarction (MI) particularly in aged rats. Methods: Chronic MI was induced in young adult (3.5 months) and aged (18 months) female Sprague-Dawley rats by ligation of the left coronary artery. Sham-operated animals of corresponding age served as controls. Heart function was measured by catheterization 4 weeks after MI. The expression of IL-1 beta, IL-6, TGF-beta-isoforms, ANF, and components of the extracellular matrix (pro-collagen I and III, colligin, MMP-2 and TIMP2) was measured by ribonuclease protection assay. Results: Aged control rats differed from young adult rats in that LV-developed pressure (LVDP) was higher (161 vs. 147 mm Hg, p < 0.05) in response to the elevated total peripheral resistance (0.71 vs. 0.47 mm Hg ml min/kg, p < 0.05). Contractility was reduced in aged controls as indicated by decreased LV dP/dt (8.106 vs. 10.606 mm Hg/s, p < 0.05). LV function was severely depressed in both MI groups (reduction in LVDP by about 35% and LV dP/dt by about 30%, increase in LVEDP to 24 mm Hg) while RVP and RV dP/dt markedly increased by about 100%. This was not different between both MI groups. ANF expression as a marker of hypertrophy was induced in both MI groups, but less pronounced in the LV of aged rats. Also, the mRNA expression pattern was qualitatively comparable, but showed gradual differences. Conclusion: These results indicate that aged rats compensate well for hemodynamic overload induced by MI. Also, the mechanisms of myocardial post-MI remodeling are comparable in young adult and aged rats. (c) 2004 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.