Genipin Inhibits LPS-Induced Inflammatory Response in BV2 Microglial Cells

被引：46

作者：

Wang, Jianjiao ^{[1
]}

Chen, Liang ^{[1
]}

Liang, Zuobin ^{[1
]}

Li, Yang ^{[1
]}

Yuan, Fei ^{[1
]}

Liu, Jie ^{[1
]}

Tian, Yu ^{[1
]}

Hao, Zhen ^{[1
]}

Zhou, Fucheng ^{[1
]}

Liu, Xuanxi ^{[1
]}

Cao, Yu ^{[1
]}

Zheng, Yongri ^{[1
]}

Li, Qingsong ^{[1
]}

机构：

[1] Harbin Med Univ, Affiliated Hosp 2, Dept Neurosurg, Harbin 150086, Heilongjiang, Peoples R China

来源：

NEUROCHEMICAL RESEARCH | 2017年 / 42卷 / 10期

基金：

中国博士后科学基金;

关键词：

Genipin; LPS; NF-kB; Nrf2; Microglia; NF-KAPPA-B; PARKINSONS-DISEASE; ACTIVATION; BRAIN; NEUROINFLAMMATION; EXPRESSION; DAMAGE;

D O I：

10.1007/s11064-017-2289-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Genipin, an aglycon of geniposide, has been reported to have anti-inflammatory effect. However, the anti-inflammatory activity of genipin on LPS-stimulated BV2 microglial cells has not been reported. In this study, we investigated the molecular mechanisms responsible for the anti-inflammatory activity of genipin both in vivo and in vitro. The levels of TNF-alpha, IL-1 beta, NO and PGE(2) were detected by ELISA. The expression of Nrf2, HO-1, and NF-kappa B were detected by western blot analysis. In vivo, genipin significantly attenuated LPS-induced memory deficit in the Morris water maze and passive avoidance tasks. Genipin also inhibited LPS-induced TNF-alpha and IL-1 beta expression in brain tissues. In vitro, our results showed that genipin inhibited LPS-induced TNF-alpha, IL-1 beta, NO and PGE(2) production in a concentration-dependent manner. Genipin also suppressed LPS-induced NF-kappa B activation. In addition, the expression of Nrf2 and HO-1 were up-regulated by treatment of genipin. Furthermore, the inhibition of genipin on inflammatory mediator production was attenuated by transfection with Nrf2 siRNA. In conclusion, genipin inhibited LPS-induced inflammatory response by activating Nrf2 signaling pathway in BV2 microglia.

引用

页码：2769 / 2776

页数：8

共 26 条

[1]

Genipin attenuates lipopolysaccharide-induced persistent changes of emotional behaviors and neural activation in the hypothalamic paraventricular nucleus and the central amygdala nucleus [J].