Pulsatile perfusion system for ex vivo investigation of biochemical pathways in intact vascular tissue

被引:46
作者
Labadie, RF
Antaki, JF
Williams, JL
Katyal, S
Ligush, J
Watkins, SC
Pham, SM
Borovetz, HS
机构
[1] UNIV PITTSBURGH, DEPT SURG, PITTSBURGH, PA 15261 USA
[2] UNIV PITTSBURGH, DEPT CELL BIOL, PITTSBURGH, PA 15261 USA
[3] UNIV PITTSBURGH, DEPT PHYSIOL, PITTSBURGH, PA 15261 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1996年 / 270卷 / 02期
关键词
control theory; electron microscopy; hemodynamics; vasomotor response;
D O I
10.1152/ajpheart.1996.270.2.H760
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We have constructed and performed initial validation of an innovative perfusion system that allows exposure of intact segments of vascular tissue to realistic physiological and hemodynamic environments ex vivo. Computer-controlled opening and closing of an in-line gate valve allows generation of arterial pressure waveforms. The control algorithm predicted resultant pressure waveforms with a high degree of accuracy (Pearson correlation coefficient > 0.97). To document vascular homeostasis ex vivo, vasomotor bioassays and morphological studies were performed. The bioassays consisted of injecting epinephrine (2 x 10(-3) mg/ml) into the perfusion system followed by acetylcholine (100 mu M) while concurrently measuring vessel diameter with a laser micrometer; significant vasomotion was measured for canine carotid arteries (n = 4) bioassayed after 1, 24, and 48 h of perfusion (P < 0.03). Additionally, human saphenous vein segments were perfused for 24 h (n = 4) and viewed with laser confocal scanning microscopy and transmission electron microscopy; photomicrographs show typical vascular morphology. We conclude that the vascular perfusion system described herein is well suited for investigating the response of intact vascular tissue to hemodynamic variables.
引用
收藏
页码:H760 / H768
页数:9
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