The Bw cells, a novel B cell population conserved in the whole genus Mus

被引:16
作者
Thiriot, Aude
Drapier, Anne-Marie
Vieira, Paulo
Fitting, Catherine
Cavaillon, Jean-Marc
Cazenave, Pierre-Andre
Rueff-Juy, Dominique
机构
[1] Inst Pasteur, Dept Immunol, Unite Immunophysiopathol Infect, F-75724 Paris, France
[2] Univ Paris 06, Paris, France
[3] Inst Pasteur, INSERM, Unite Dev Lymphocytes, Dept Immunol, F-75724 Paris, France
[4] Inst Pasteur, Unite Cytokines & Inflammat, Dept Infect & Epidemiol, Paris, France
[5] CNRS, Paris, France
[6] Inst Pasteur, Dept Immunol, Unite Biol Populat Lymphocytaires, F-75724 Paris, France
关键词
D O I
10.4049/jimmunol.179.10.6568
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In common laboratory mouse strains, which are derived from the crossing between three subspecies, peritoneal B cells are enriched in B-1a cells characterized by the CD5(+)Mac-l(+)B220(low)IgM(high)IgD(low)CD43(+)CD9(+) phenotype. Intriguingly in other vertebrates, CD5(+)Mac-l(+) cells have never been found in a specific anatomic site. To ascertain the peculiarity of the CD5(+) peritoneal B cells in laboratory mice, we analyzed the peritoneal B cell subsets in 9 inbred and 39 outbred wild-derived mouse strains belonging to 13 different species/subspecies. We found that most of these strains do not have the CD5(+) B-1a cell population. However, all of these strains including classical laboratory mouse strains, have variable proportions of a novel B cell population: Bw, which is characterized by a unique phenotype (CD5(-)Mac-1(+)B220(high)IgM(high)IgD high CD43(-)CD9(-)) and is not restricted to the peritoneal cavity. Bw cells are also distinct from both B-1 and B-2 cells from a functional point of view both by proliferative responses, cytokine secretion and Ab synthesis. Moreover, transfer experiments show that bone marrow and fetal liver cells from wild mice can give rise to Bw cells in alymphoid mice. The conservation of this B cell population, but not of the CD5(+) B-1a, during evolution of the genus Mus, its readiness to respond to TLR ligands and to produce high concentration of autoantibodies suggest that Bw cells play a key role in innate immunity.
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页码:6568 / 6578
页数:11
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