Monomolecular layer formation of ferritin molecules on an amphiphilic cyclodextrin derivative at the air/water interface

被引:6
作者
Kobayashi, K
Ishii, N
Sasabe, H
Knoll, W
机构
[1] Minist Int Trade & Ind, Agcy Ind Sci & Technol, Natl Inst Biosci & Human Technol, Biophys Chem Lab, Tsukuba, Ibaraki 3058566, Japan
[2] Inst Phys & Chem Res, Frontier Res Program, Wako, Saitama 3510198, Japan
[3] Max Planck Inst Polymerforsch, D-55128 Mainz, Germany
关键词
monolayer; ferritin; cyclodextrin derivative; surface pressure; transmission electron microscopy;
D O I
10.1271/bbb.65.176
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A monomolecular layer of ferritin molecules was formed by adsorption from the subphase onto a Langmuir film of an amphiphilic beta -cyclodextrin (beta -CD) derivative at the air/water interface. The course of the adsorption of ferritin molecules was monitored by measuring the surface pressure and the resulting film was observed by transmission electron microscopy (TEM), These results show the potential of the amphiphilic CD derivative to work as a milder template for protein molecules at the air/water interface.
引用
收藏
页码:176 / 179
页数:4
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