Brn3a regulation of TrkA/NGF receptor expression in developing sensory neurons

被引:54
作者
Ma, L
Lei, L
Eng, R
Turner, E
Parada, LF
机构
[1] Univ Texas, SW Med Ctr, Ctr Dev Biol, Dallas, TX 75390 USA
[2] Univ Texas, SW Med Ctr, Kent Waldrep Fdn, Ctr Basic Res Nerve Growth & Regenerat, Dallas, TX USA
[3] Univ Calif San Diego, San Diego VA Med Ctr, La Jolla, CA 92093 USA
[4] Univ Calif San Diego, Dept Psychiat, La Jolla, CA 92093 USA
来源
DEVELOPMENT | 2003年 / 130卷 / 15期
关键词
TrkA; Brn3a; sensory neuron; transcriptional regulation; Bax;
D O I
10.1242/dev.00582
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The TrkA/NGF receptor is essential for the survival and differentiation of sensory neurons. The molecular mechanisms regulating tissue and stage-specific expression of TrkA are largely unknown. The Brn3a POU-domain transcription factor has been implicated in the development of the PNS and proposed as a transcription regulator for TrkA. The molecular mechanisms underlying the regulation of TrkA by Brn3a is unclear. In this study, we provide genetic, transgenic and biochemical evidence that Brn3a binds to novel, specific sites in the 457 bp enhancer that regulates TrkA expression in embryonic sensory neurons. We employ Bax-knockout mice, in which sensory neurons no longer require neurotrophins for survival, to uncouple TrkA-dependent cell death from downregulation of TrkA expression. In addition, when mutagenized, the novel Brn3a-binding sites identified fail to drive appropriate reporter transgene expression in sensory neurons. Thus, TrkA, a gene that is crucial for the differentiation and survival of sensory nociceptive neurons, requires Brn3a to maintain normal transcriptional activity.
引用
收藏
页码:3525 / 3534
页数:10
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