Microarray-based genomic selection for high-throughput resequencing

被引：260

作者：

Okou, David T.

Steinberg, Karyn Meltz

Middle, Christina

Cutler, David J.

Albert, Thomas J.

Zwick, Michael E.

机构：

[1] Emory Univ, Sch Med, Dept Human Genet, Atlanta, GA 30322 USA

[2] Emory Univ, Program Populat Biol Ecol & Evolut, Atlanta, GA 30322 USA

[3] NimbleGen Syst Inc, Madison, WI 53711 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 11期

关键词：

D O I：

10.1038/NMETH1109

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a general method, microarray-based genomic selection (MGS), capable of selecting and enriching targeted sequences from complex eukaryotic genomes without the repeat blocking steps necessary for bacterial artificial chromosome (BAC)-based genomic selection. We demonstrate that large human genomic regions, on the order of hundreds of kilobases, can be enriched and resequenced with resequencing arrays. MGS, when combined with a next-generation resequencing technology, can enable large-scale resequencing in singleinvestigator laboratories.

引用

页码：907 / 909

页数：3

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