Genetic diversity of Phytophthora infestans in the Northern Andean region

被引：40

作者：

Cardenas, Martha ^{[1
]}

Grajales, Alejandro ^{[1
]}

Sierra, Roberto ^{[1
]}

Rojas, Alejandro ^{[1
]}

Gonzalez-Almario, Adriana ^{[1
]}

Vargas, Angela ^{[1
]}

Marin, Mauricio ^{[2
]}

Fermin, Gustavo ^{[3
]}

Lagos, Luz E. ^{[4
]}

Gruenwald, Niklaus J. ^{[5
]}

Bernal, Adriana ^{[1
]}

Salazar, Camilo ^{[6
,7
]}

Restrepo, Silvia ^{[1
]}

机构：

[1] Univ Los Andes, Bogota, Colombia

[2] Univ Nacl Colombia, Medellin, Antioquia, Colombia

[3] Univ Los Andes, Merida, Venezuela

[4] Univ Narino, Pasto, Narino, Colombia

[5] ARS, USDA, Corvallis, OR USA

[6] Smithsonian Trop Res Inst, Panama City, Panama

[7] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England

来源：

BMC GENETICS | 2011年 / 12卷

关键词：

LATE BLIGHT; POPULATION-STRUCTURE; CENTRAL MEXICO; PHYLOGENETIC ANALYSIS; MAXIMUM-LIKELIHOOD; STATISTICAL-METHOD; CULTIVATED POTATO; SOLANACEOUS HOSTS; DNA POLYMORPHISM; EFFECTOR AVR3A;

D O I：

10.1186/1471-2156-12-23

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: Phytophthora infestans (Mont.) de Bary, the causal agent of potato late blight, is responsible for tremendous crop losses worldwide. Countries in the northern part of the Andes dedicate a large proportion of the highlands to the production of potato, and more recently, solanaceous fruits such as cape gooseberry (Physalis peruviana) and tree tomato (Solanum betaceum), all of which are hosts of this oomycete. In the Andean region, P. infestans populations have been well characterized in Ecuador and Peru, but are poorly understood in Colombia and Venezuela. To understand the P. infestans population structure in the Northern part of the Andes, four nuclear regions (ITS, Ras, beta-tubulin and Avr3a) and one mitochondrial (Cox1) region were analyzed in isolates of P. infestans sampled from different hosts in Colombia and Venezuela. Results: Low genetic diversity was found within this sample of P. infestans isolates from crops within several regions of Colombia and Venezuela, revealing the presence of clonal populations of the pathogen in this region. We detected low frequency heterozygotes, and their distribution patterns might be a consequence of a high migration rate among populations with poor effective gene flow. Consistent genetic differentiation exists among isolates from different regions. Conclusions: The results here suggest that in the Northern Andean region P. infestans is a clonal population with some within-clone variation. P. infestans populations in Venezuela reflect historic isolation that is being reinforced by a recent self-sufficiency of potato seeds. In summary, the P. infestans population is mainly shaped by migration and probably by the appearance of variants of key effectors such as Avr3a.

引用

页数：13

共 51 条

[1] Another look at the origin of late blight of potatoes, tomatoes, and pear melon in the Andes of South America [J].