Arabidopsis thaliana flavoprotein AtHAL3a catalyzes the decarboxylation of 4′-phosphopantothenoylcysteine to 4′-phosphopantetheine, a key step in coenzyme A biosynthesis

被引:44
作者
Kupke, T
Hernández-Acosta, P
Steinbacher, S
Culiáñez-Macià, FA
机构
[1] Univ Tubingen, Lehrstuhl Mikrobielle Genet, D-72076 Tubingen, Germany
[2] Univ Politecn Valencia, Inst Mol & Celular Plantas, CSIC, Valencia 46022, Spain
[3] Max Planck Inst Biochem, Abt Strukturforsch, D-82152 Planegg Martinsried, Germany
关键词
D O I
10.1074/jbc.M100776200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arabidopsis thaliana flavoprotein AtHAL3a is related to plant growth and salt and osmotic tolerance. AtHAL3a shows sequence homology to the bacterial flavoproteins EpiD and Dfp. EpiD, Dfp, and AtHAL3a are members of the homo-oligomeric flavin-containing Cys decarboxylase (HFCD) protein family. We demonstrate that AtHAL3a catalyzes the decarboxylation of (R)-4 ' -phospho-N-pantothenoylcysteine to 4 ' -phosphopantetheine. This key step in coenzyme A biosynthesis is catalyzed in bacteria by the Dfp proteins. Exchange of His-90 of AtHAL3a for Asn led to complete inactivation of the enzyme. Dfp and AtHAL3a are characterized by a shortened substrate binding clamp compared with EpiD. Exchange of the cysteine residue of the conserved ACGD motif of this binding clamp resulted in loss of (R)-4 ' -phospho-N-pantothenoylcysteine decarboxylase activity. Based on the crystal structures of EpiD H67N with bound substrate peptide and of AtHAL3a, we present a model for the binding of (R)-4 ' -phospho-N-pantothenoylcysteine to AtHAL3a.
引用
收藏
页码:19190 / 19196
页数:7
相关论文
共 29 条
  • [1] Abiko Y., 1975, Metabolism of sulphur compounds., P1
  • [2] The X-ray structure of the FMN-binding protein AtHal3 provides the structural basis for the activity of a regulatory subunit involved in signal transduction
    Albert, A
    Martínez-Ripoll, M
    Espinosa-Ruiz, A
    Yenush, L
    Culiáñez-Macià, FA
    Serrano, R
    [J]. STRUCTURE, 2000, 8 (09) : 961 - 969
  • [3] Crystal structure of the peptidyl-cysteine decarboxylase EpiD complexed with a pentapeptide substrate
    Blaesse, M
    Kupke, T
    Huber, R
    Steinbacher, S
    [J]. EMBO JOURNAL, 2000, 19 (23) : 6299 - 6310
  • [4] Sodium transport and salt tolerance in plants
    Blumwald, E
    [J]. CURRENT OPINION IN CELL BIOLOGY, 2000, 12 (04) : 431 - 434
  • [5] Clotet J, 1999, MOL CELL BIOL, V19, P2408
  • [6] The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase
    de Nadal, E
    Clotet, J
    Posas, F
    Serrano, R
    Gomez, N
    Ariño, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (13) : 7357 - 7362
  • [7] DICOMO CJ, 1995, GENETICS, V139, P95
  • [8] DUPRE S, 1975, ITAL J BIOCHEM, V24, P369
  • [9] Arabidopsis thaliana AtHAL3:: a flavoprotein related to salt and osmotic tolerance and plant growth
    Espinosa-Ruiz, A
    Bellés, JM
    Serrano, R
    Culiáñez-Macià, FA
    [J]. PLANT JOURNAL, 1999, 20 (05) : 529 - 539
  • [10] COENZYME-A BIOSYNTHESIS IN PLANTS - PARTIAL-PURIFICATION AND CHARACTERIZATION OF PANTOTHENATE KINASE FROM SPINACH
    FALK, KL
    GUERRA, DJ
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 301 (02) : 424 - 430