A VALIDATED HPLC-DAD-MS METHOD FOR IDENTIFYING AND DETERMINING THE BIOACTIVE COMPONENTS OF TWO KINDS OF LUOBUMA

被引:9
作者
Shi, Junyou [1 ,2 ]
Li, Guoliang [1 ,2 ]
Zhang, Rui [1 ,2 ]
Zheng, Jie [1 ,2 ]
Suo, Yourui [1 ]
You, Jinmao [1 ]
Liu, Yong-jun [1 ]
机构
[1] Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
关键词
Apoacynum venetum L; flavonoids; liquid chromatography-mass spectrometry; Poacynum hendersonii; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; APOCYNUM-VENETUM LEAVES; AQUEOUS EXTRACTS; QUERCETIN; FLAVONOIDS; RATS; POLYPHENOLS; CONSTITUENTS; TEAS;
D O I
10.1080/10826076.2011.546173
中图分类号
Q5 [生物化学];
学科分类号
070307 [化学生物学];
摘要
A rapid and effective HPLC-DAD-MS method for quantitatively identifying the flavonoids of Apoacynum venetum L and Poacynum hendersonii has been developed. With this method, all the main components were well separated. In Apoacynum venetum L, the main components are chlorogenic acid, hyperoside, isoquercitrin, and 6''-O-malonylhyperoside, while those of Poacynum hendersonii are quercetin3-O-sophoroside, isoquercitrin and 6''-O-malonylhyperoside. The experimental results showed that the developed approach is accurate, reproducible, and applicable for quantifying the bioactive components of Apoacynum venetum L and Poacynum hendersonii.
引用
收藏
页码:537 / 547
页数:11
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