The advantage of mucosal immunization for polysaccharide-specific memory responses in early life

被引:26
作者
Bjarnarson, SP
Jakobsen, H
Del Giudice, G
Trannoy, E
Siegrist, CA
Jonsdottir, I [1 ]
机构
[1] Landspitali Univ Hosp, Dept Immunol, IS-101 Reykjavik, Iceland
[2] Chiron Srl, IRIS, Siena, Italy
[3] Aventis Pasteur, Marcy Letoile, France
[4] Univ Geneva, WHO, Collaborating Ctr Neonatal Vaccinol & Immunol, Geneva, Switzerland
[5] Univ Iceland, Fac Med, Reykjavik, Iceland
关键词
neonatal vaccination; B cell memory; mucosal; pneumococcal polysaccharide; pneumococcal conjugate;
D O I
10.1002/eji.200425850
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The aim of vaccination is to rapidly elicit protective immunity and generate memory for sustained protection. We studied the induction and persistence of polysaccharide (PS)specific memory in neonatal and infant mice primed with pneumococcal conjugate (Pnc1-TT) by assessing the response to native pneumococcal PS (PPS-1), the kinetics of the PPS-1-specific IgG response to a second Pnc1-TT dose and affinity maturation. A subcutaneous (s.c.) Pncl-TT booster induced a rapid increase in PPS-1-specific IgG, indicating efficient priming for memory by a single dose of Pncl-TT already at I week of age. High levels were maintained for > 12 weeks. However, a PPS-1 booster induced no response in neonatal or infant mice. The adjuvant LT-K63 significantly enhanced the IgG response and affinity to Pncl-TT by both the s.c. and the intranasal (i.n.) route in all age groups. In neonatal and infant mice, PPS-1 and LT-K63 induced a booster response only when given i.n. following either s.c. or i.n. priming with Pncl-TT and LT-K63. In contrast, PPS-1 with or without LT-K63 administered s.c. compromised the ongoing PPS-1-specific response elicited in neonatal mice by either s.c. or i.n. priming with Pnc1-TT and LT-K63. These results demonstrate the advantage of the mucosal route for elicitation of PS-specific memory responses in early life.
引用
收藏
页码:1037 / 1045
页数:9
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