High-throughput in vivo analysis of gene expression in Caenorhabditis elegans

被引:285
作者
Hunt-Newbury, Rebecca
Viveiros, Ryan
Johnsen, Robert
Mah, Allan
Anastas, Dina
Fang, Lily
Halfnight, Erin
Lee, David
Lin, John
Lorch, Adam
McKay, Sheldon
Okada, H. Mark
Pan, Jie
Schulz, Ana K.
Tu, Domena
Wong, Kim
Zhao, Z.
Alexeyenko, Andrey
Burglin, Thomas
Sonnhammer, Eric
Schnabel, Ralf
Jones, Steven J.
Marra, Marco A.
Baillie, David L.
Moerman, Donald G. [1 ]
机构
[1] Univ British Columbia, Dept Zool, Vancouver, BC V5Z 1M9, Canada
[2] Univ British Columbia, Michael Smith Labs, Vancouver, BC V5Z 1M9, Canada
[3] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada
[4] British Columbia Canc Agcy, Genome Sci Ctr, Burnaby, BC, Canada
[5] Tech Univ Carolo Wilhelmina Braunschweig, Inst Genet, Braunschweig, Germany
[6] Karolinska Inst, Ctr Genom & Bioinformat, Stockholm, Sweden
[7] Karolinska Inst, Dept Biosci, Huddinge, Sweden
关键词
CELL LINEAGE; C-ELEGANS; FUNCTIONAL GENOMICS; NERVOUS-SYSTEM; MUSCLE; NEMATODE; TRANSCRIPTION; WORMBASE; SEQUENCE; DATABASE;
D O I
10.1371/journal.pbio.0050237
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using DNA sequences 59 to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism. GFP-expressing wild-type animals were analyzed at each stage of development from embryo to adult. We have identified 59 DNA regions regulating expression at all developmental stages and in 38 different cell and tissue types in this organism. Among the regulatory regions identified are sequences that regulate expression in all cells, in specific tissues, in combinations of tissues, and in single cells. Most of the genes we have examined in C. elegans have human orthologs. All the images and expression pattern data generated by this project are available at WormAtlas (http://gfpweb.aecom.yu.edu/index) and through WormBase (http://www.wormbase.org).
引用
收藏
页码:1981 / 1997
页数:17
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