Visualization of endogenous BMP signaling during Xenopus development

被引：37

作者：

Kurata, T

Nakabayashi, J

Yamamoto, TS

Mochii, M

Ueno, N

机构：

[1] Natl Inst Basic Biol, Dept Dev Biol, Div Morphogenesis, Okazaki, Aichi 4448585, Japan

[2] Grad Univ Adv Studies, Sch Life Sci, Dept Mol Biomech, Okazaki, Aichi 4448585, Japan

来源：

DIFFERENTIATION | 2001年 / 67卷 / 1-2期

基金：

日本学术振兴会;

关键词：

BMP; Smad; morphogen; gradient; Xenopus laevis;

D O I：

10.1046/j.1432-0436.2001.067001033.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The TGF-beta superfamily of growth factors is known to transmit signals to the nucleus mainly through the Smads, intracellular signaling components that are highly conserved from nematodes to humans. The signaling activity of the Smads is regulated by their ligand-stimulated phosphorylation through Ser/Thr kinase receptors. Here, to examine the in vivo role of BMP, we investigated the spatio-temporal activation of BMP-regulated signals during Xenopus development, using a polyclonal antibody that specifically recognizes the phosphorylated form of BMP-regulated Smads. BMP signaling was observed uniformly in embryos as early as stage 7, but was restricted to the ventral side of the embryo at the late blastula stage, supporting the proposed role of BMP4 as a ventralizing factor in Xenopus embryos. In addition, localized staining was detected in several developing organs, consistent with the predicted function of BMP family members in organogenesis.

引用

页码：33 / 40

页数：8

共 37 条

[1] Wnt signaling in Xenopus embryos inhibits Bmp4 expression and activates neural development [J].