Demethoxylation of lignin-model compounds with enzyme extracts from Gloeophilum trabeum

被引：21

作者：

Lopretti, M

Cabella, D

Morais, J

Rodrigues, A ^{[1
]}

机构：

[1] Univ Porto, Sch Engn, Lab Separat & React Engn, P-4099 Oporto, Portugal

[2] Univ Republica, Fac Vet, Fac Ciencias, Montevideo, Uruguay

来源：

PROCESS BIOCHEMISTRY | 1998年 / 33卷 / 06期

关键词：

demethoxylation; lignin-model compounds; activity; enzyme extracts; Gloeophilum trabeum;

D O I：

10.1016/S0032-9592(98)00028-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lignin demethoxylation was studied using enzyme extracts from Gloeophilum trabeum. The synthesis of enzyme complexes in semisolid cultures is described together with the incorporation of Fe-55 to identify metalproteins. The demethoxylation activity of enzyme extracts were tested with lignin-model compounds: veratric alcohol; veratric aldehyde; anisoin. Demethoxylated products were identified by thin layer chromatography and reverse phase high-pressure liquid chromatography. Methanol was identified as a reaction product when vanillin and syringic acid were used as substrates. Demethoxylation of lignin by fungi can be an important step for the production of biodegradable polymers such as polyurethanes, as a result of the transformation of methoxylated substrates into hydroxylated products. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：657 / 661

页数：5

共 18 条

[1] VANILLIC ACID METABOLISM BY SPOROTRICHUM-PULVERULENTUM - EVIDENCE FOR DEMETHOXYLATION BEFORE RING-CLEAVAGE [J].