Analysis of glucosinolates from broccoli and other cruciferous vegetables by hydrophilic interaction liquid chromatography

被引:63
作者
Troyer, JK
Stephenson, KK
Fahey, JW
机构
[1] Johns Hopkins Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA
[2] Brassica Protect Prod, Baltimore, MD 21202 USA
关键词
vegetables; food analysis; glucosinolates;
D O I
10.1016/S0021-9673(01)00842-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
While methods for the identification and quantification of total plant glucosinolate content typically utilize desulfation of glucosinolates followed by reversed-phase chromatography, the analysis of intact glucosinolates has been problematic. Hydrophilic interaction chromatography offers a novel method for analyzing intact glucosinolates and when performed along with ion-pair reversed-phase chromatography offers a powerful and complementary method for glucosinolate analysis. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:299 / 304
页数:6
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