ATP stimulated cyclic AMP formation in bovine chromaffin cells is enhanced by neuropeptide Y

被引：12

作者：

Zhang, PJ

Zheng, JL

Bradley, ME

Hexum, TD ^{[1
]}

机构：

[1] Univ Nebraska, Dept Pharmacol, Omaha, NE USA

[2] Creighton Univ, Sch Med, Dept Pharmacol, Omaha, NE USA

来源：

PEPTIDES | 2001年 / 22卷 / 03期

关键词：

receptor; crosstalk; G protein; chromaffin cell; signaling;

D O I：

10.1016/S0196-9781(01)00354-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP increases cAMP formation in bovine chromaffin cells, EC50 = 7.1 X 10(-6) M. NPY, EC50 = 4.1 x 10(-8) M, increases the efficacy of ATP (1.5-2 fold). Inclusion of the selective Y1 receptor antagonist 1229U91 produced a decrease in NPY potency (EC50 = 2.7 x 10(-7) M). PTX pretreatment did not abolish either the effect of ATP nor the enhancement by NPY. NPY could also enhance the ability of angiotensin and bradykinin to increase cAMP formation. The selective phospholipase C inhibitor, U73122, and the selective protein kinase C inhibitors, bisindoiylmaleimide I and RO-31-8425, were effective inhibitors of the enhancing effect of NPY. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：439 / 444

页数：6

共 15 条

[1]

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[2] HIGH-AFFINITY NEUROPEPTIDE-Y RECEPTOR ANTAGONISTS [J].