Microsatellite markers for Vaccinium from EST and genomic libraries

被引：62

作者：

Boches, PS

Bassil, NV

Rowland, LJ

机构：

[1] US EPA, NCGR, Corvallis, OR 97333 USA

[2] USDA ARS, Fruit Lab, Beltsville, MD 20705 USA

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 03期

关键词：

blueberry; EST; microsatellite; PCR; SSR; Vaccinium;

D O I：

10.1111/j.1471-8286.2005.01025.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present 30 microsatellite loci isolated from expressed sequence tag (EST) and genomic libraries in Vaccinium corymbosum L. Allele number per locus in 11 tetraploid and one diploid V. corymbosum accessions ranged from two to 15 (mean = 8.16) in 24 single-locus simple sequence repeats (SSRs). Cross-species amplification in a panel of 12 species representing nine sections ranged from 30 to 100% (mean = 83%).

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收藏

页码：657 / 660

页数：4

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