Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence

被引：39

作者：

Dumont, P ^{[1
]}

Royer, V ^{[1
]}

Pascal, T ^{[1
]}

Dierick, JF ^{[1
]}

Chainiaux, F ^{[1
]}

Frippiat, C ^{[1
]}

de Magalhaes, JP ^{[1
]}

Eliaers, F ^{[1
]}

Remacle, J ^{[1
]}

Toussaint, O ^{[1
]}

机构：

[1] Fac Univ Notre Dame Paix, Dept Biol, Lab Cellular Biochem & Biol, B-5000 Namur, Belgium

来源：

FEBS LETTERS | 2001年 / 502卷 / 03期

关键词：

telomere; aging; senescence; oxidative stress; H2O2;

D O I：

10.1016/S0014-5793(01)02679-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 muM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings; (PD). After subcytotoxic 160 muM H2O2 stress or five repeated 30 muM t-BHP stresses along the same PD, respectively a 322 +/- 55 and 380 +/- 129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：109 / 112

页数：4

共 13 条

[1] TERT-BUTYL HYDROPEROXIDE-MEDIATED DNA-BASE DAMAGE IN CULTURED-MAMMALIAN-CELLS [J].

ALTMAN, SA ;

ZASTAWNY, TH ;

RANDERS, L ;

LIN, ZL ;

LUMPKIN, JA ;

REMACLE, J ;

DIZDAROGLU, M ;

RAO, G .

MUTATION RESEARCH, 1994, 306 (01) :35-44

[2] Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts:: p53 and Rb control G1 arrest but not cell replication [J].

Chen, QM ;

Bartholomew, JC ;

Campisi, J ;

Acosta, M ;

Reagan, JD ;

Ames, BN .

BIOCHEMICAL JOURNAL, 1998, 332 :43-50

[3] CELLULAR SENESCENCE AND DNA-SYNTHESIS - THYMIDINE INCORPORATION AS A MEASURE OF POPULATION AGE IN HUMAN DIPLOID CELLS [J].

CRISTOFALO, VJ ;

SHARF, BB .

EXPERIMENTAL CELL RESEARCH, 1973, 76 (02) :419-427

[4] A BIOMARKER THAT IDENTIFIES SENESCENT HUMAN-CELLS IN CULTURE AND IN AGING SKIN IN-VIVO [J].

DIMRI, GP ;

LEE, XH ;

BASILE, G ;

ACOSTA, M ;

SCOTT, C ;

ROSKELLEY, C ;

MEDRANO, EE ;

LINSKENS, M ;

RUBELJ, I ;

PEREIRASMITH, O ;

PEACOCKE, M ;

CAMPISI, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9363-9367

[5] Induction of replicative senescence biomarkers by sublethal oxidative stresses in normal human fibroblast [J].

Dumont, P ;

Burton, M ;

Chen, QM ;

Gonos, ES ;

Frippiat, C ;

Mazarati, JB ;

Eliaers, F ;

Remacle, J ;

Toussaint, O .

FREE RADICAL BIOLOGY AND MEDICINE, 2000, 28 (03) :361-373

[6] Subcytotoxic H2O2 stress triggers a release of transforming growth factor-β1, which induces biomarkers of cellular senescence of human diploid fibroblasts [J].

Frippiat, C ;

Chen, QM ;

Zdanov, S ;

Magalhaes, JP ;

Remacle, J ;

Toussaint, O .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (04) :2531-2537

[7] SERIAL CULTIVATION OF HUMAN DIPLOID CELL STRAINS [J].

HAYFLICK, L ;

MOORHEAD, PS .

EXPERIMENTAL CELL RESEARCH, 1961, 25 (03) :585-+

[8] Preferential accumulation of single-stranded regions in telomeres of human fibroblasts [J].

Petersen, S ;

Saretzki, G ;

von Zglinicki, T .

EXPERIMENTAL CELL RESEARCH, 1998, 239 (01) :152-160

[9] DEFINING CELLULAR SENESCENCE IN IMR-90 CELLS - A FLOW CYTOMETRIC ANALYSIS [J].

SHERWOOD, SW ;

RUSH, D ;

ELLSWORTH, JL ;

SCHIMKE, RT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :9086-9090

[10] Cellular and molecular mechanisms of stress-induced premature senescence (SIPS) of human diploid fibroblasts and melanocytes [J].

Toussaint, O ;

Medrano, EE ;

von Zglinicki, T .

EXPERIMENTAL GERONTOLOGY, 2000, 35 (08) :927-945

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