Molecular cloning and functional characterization of rat Δ-6 fatty acid desaturase

被引：112

作者：

Aki, T ^{[1
]}

Shimada, Y ^{[1
]}

Inagaki, K ^{[1
]}

Higashimoto, H ^{[1
]}

Kawamoto, S ^{[1
]}

Shigeta, S ^{[1
]}

Ono, K ^{[1
]}

Suzuki, O ^{[1
]}

机构：

[1] Hiroshima Univ, Dept Mol Biotechnol, Higashihiroshima 7398527, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 255卷 / 03期

关键词：

D O I：

10.1006/bbrc.1999.0235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian cDNA fragments putatively encoding amino acid sequences characteristic of the fatty acid desaturase were obtained using expressed sequence tag (EST) sequence informations. These fragments were subsequently used to screen a rat liver cDNA library, yielding a 1573-bp clone. Expression of DNA fragment containing either of two possible open reading frames (nucleotide numbers 97-1431 and 148-1431) of the isolated clone in yeast led to the accumulation of gamma-linolenic acid in the presence of exogenous linoleic acid. In this system, the addition of alpha-linolenic acid also resulted in the accumulation of its Delta-6 desaturated product whereas dihomo-gamma-linolenic acid failed to be a substrate. These results indicate that the protein encoded by the rat cDNA is Delta-6 fatty acid desaturase, and the first 17 amino acids corresponding to the coding region 97-147 of the clone are not required to function in yeast. (C) 1999 Academic Press.

引用

页码：575 / 579

页数：5

共 24 条

[11] POSSIBLE ROLE OF FLANKING NUCLEOTIDES IN RECOGNITION OF THE AUG INITIATOR CODON BY EUKARYOTIC RIBOSOMES [J].

KOZAK, M .

NUCLEIC ACIDS RESEARCH, 1981, 9 (20) :5233-5252

[12]

LI BY, 1994, J LIPID RES, V35, P1869

[13] THE LEUKOTRIENES IN ALLERGY AND INFLAMMATION [J].

MARX, JL .

SCIENCE, 1982, 215 (4538) :1380-1383

[14] Functional identification of a fatty acid Δ5 desaturase gene from Caenorhabditis elegans [J].

Michaelson, LV ;

Napier, JA ;

Lewis, M ;

Griffiths, G ;

Lazarus, CM ;

Stobart, AK .

FEBS LETTERS, 1998, 439 (03) :215-218

[15] Isolation of a Δ5-fatty acid desaturase gene from Mortierella alpina [J].

Michaelson, LV ;

Lazarus, CM ;

Griffiths, G ;

Napier, JA ;

Stobart, AK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (30) :19055-19059

[16]

Napier JA, 1998, BIOCHEM J, V330, P611

[17]

Napier JA, 1997, BIOCHEM J, V328, P717

[18] PURIFICATION AND PARTIAL CHARACTERIZATION OF LINOLEOYL-COA DESATURASE FROM RAT-LIVER MICROSOMES [J].

OKAYASU, T ;

NAGAO, M ;

ISHIBASHI, T ;

IMAI, Y .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1981, 206 (01) :21-28

[19] PROSTAGLANDINS AND THROMBOXANES [J].

SAMUELSSON, B ;

GOLDYNE, M ;

GRANSTROM, E ;

HAMBERG, M ;

HAMMARSTROM, S ;

MALMSTEN, C .

ANNUAL REVIEW OF BIOCHEMISTRY, 1978, 47 :997-1029

[20] Expression of a borage desaturase cDNA containing an N-terminal cytochrome b(5) domain results in the accumulation of high levels of Delta(6)-desaturated fatty acids in transgenic tobacco [J].

Sayanova, O ;

Smith, MA ;

Lapinskas, P ;

Stobart, AK ;

Dobson, G ;

Christie, WW ;

Shewry, PR ;

Napier, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (08) :4211-4216

← 1 2 3 →