Variation of high mannose chains of Tamm-Horsfall glycoprotein confers differential binding to type 1-fimbriated Escherichia coli

被引:32
作者
Cavallone, D
Malagolini, N
Monti, A
Wu, XR
Serafini-Cessi, F
机构
[1] Univ Bologna, Dept Expt Pathol, I-40126 Bologna, Italy
[2] NYU, Sch Med, Dept Urol, New York, NY 10016 USA
[3] NYU, Sch Med, Dept Microbiol, New York, NY 10016 USA
关键词
D O I
10.1074/jbc.M308821200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tamm-Horsfall glycoprotein (THP), the most abundant protein in mammalian urine, has been implicated in defending the urinary tract against infections by type 1-fimbriated Escherichia coli. Recent experimental evidence indicates that the defensive capability of THP relies on its single high mannose chain, which binds to E. coli FimH lectin and competes with mannosylated uroplakin receptors on the bladder surface. Here we describe several major differences, on both structural and functional levels, between human THP (hTHP) and pig THP (pTHP). pTHP contains a much higher proportion (47%) of Man(5)GlcNAc(2) than does hTHP (8%). FimH-expressing E. coli adhere to monomeric pTHP at an approximately 3-fold higher level than to monomeric hTHP. This suggests that the shorter high mannose chain ( Man5GlcNAc2) is a much better binder for FimH than the longer chains (Man(6-7)GlcNAc(2)) and that pTHP is a more potent urinary defense factor than hTHP. In addition, unlike hTHP whose polyantennary glycans are exclusively capped by sialic acid and sulfate groups, those of pTHP are also terminated by Galalpha1,3Gal epitope. This is consistent with the fact that the outer medulla of pig kidney expresses the alpha1,3-galactosyltransferase, which is completely absent in human kidney. Finally, pTHP is more resistant to leukocyte elastase hydrolysis than hTHP, thus explaining why pTHP is much less prone to urinary degradation than hTHP. These results demonstrate for the first time that the species variations of the glycomoiety of THP can lead to the differential binding of THP to type 1-fimbriated E. coli and that the differences in high mannose processing may reflect species-specific adaptation of urinary defenses against E. coli infections.
引用
收藏
页码:216 / 222
页数:7
相关论文
共 54 条
[1]   PROPERTIES OF A GLYCOPEPTIDE ISOLATED FROM HUMAN TAMM-HORSFALL GLYCOPROTEIN - INTERACTION WITH LEUCOAGGLUTININ AND ANTI-(HUMAN TAMM-HORSFALL GLYCOPROTEIN) ANTIBODIES [J].
ABBONDANZA, A ;
FRANCESCHI, C ;
LICASTRO, F ;
SERAFINICESSI, F .
BIOCHEMICAL JOURNAL, 1980, 187 (02) :525-528
[2]   BACTERIAL ADHERENCE - ADHESION-RECEPTOR INTERACTIONS MEDIATING THE ATTACHMENT OF BACTERIA TO MUCOSAL SURFACES [J].
BEACHEY, EH .
JOURNAL OF INFECTIOUS DISEASES, 1981, 143 (03) :325-345
[3]   SOME FACTORS AFFECTING PRODUCTION, BY CULTURED BABY-HAMSTER KIDNEY CELLS, OF BHK GLYCOPROTEIN .1. WHICH CROSS-REACTS IMMUNOLOGICALLY WITH TAMM-HORSFALL GLYCOPROTEIN [J].
BLOOMFIELD, FJ ;
DUNSTAN, DR ;
FOSTER, CL ;
SERAFINICESSI, F ;
MARSHALL, RD .
BIOCHEMICAL JOURNAL, 1977, 164 (01) :41-&
[4]   Salt-precipitation method does not isolate to homogeneity Tamm-Horsfall glycoprotein from urine of proteinuricz patients and pregnant women [J].
Cavallone, D ;
Malagolini, N ;
Frascà, GM ;
Stefoni, S ;
Serafini-Cessi, F .
CLINICAL BIOCHEMISTRY, 2002, 35 (05) :405-410
[5]   Mechanism of release of urinary Tamm-Horsfall glycoprotein from the kidney GPI-anchored counterpart [J].
Cavallone, D ;
Malagolini, N ;
Serafini-Cessi, F .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 280 (01) :110-114
[6]   STRUCTURAL-ANALYSIS OF THE PREPONDERANT HIGH-MANNOSE OLIGOSACCHARIDE OF HUMAN TAMM-HORSFALL GLYCOPROTEIN [J].
DALLOLIO, F ;
DEKANTER, FJJ ;
VANDENEIJNDEN, DH ;
SERAFINICESSI, F .
CARBOHYDRATE RESEARCH, 1988, 178 :327-332
[7]   DEVELOPMENT OF A RADIOIMMUNOASSAY FOR TAMM-HORSFALL GLYCOPROTEIN IN SERUM [J].
DAWNAY, A ;
MCLEAN, C ;
CATTELL, WR .
BIOCHEMICAL JOURNAL, 1980, 185 (03) :679-687
[8]   Virulence-associated factors of uropathogenic Escherichia coli strains isolated from pigs [J].
de Brito, BG ;
Leite, DD ;
Linhares, REC ;
Vidotto, MC .
VETERINARY MICROBIOLOGY, 1999, 65 (02) :123-132
[9]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[10]   PROTEIN, IMMUNOLOGICALLY SIMILAR TO TAMM-HORSFALL GLYCOPROTEIN, PRODUCED BY CULTURED BABY HAMSTER KIDNEY-CELLS [J].
DUNSTAN, DR ;
GRANT, AMS ;
MARSHALL, RD ;
NEUBERGER, A .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1974, 186 (1085) :297-+