Interferon-like activity produced by anterior kidney leucocytes of rainbow trout stimulated in vitro by infectious hematopoietic necrosis virus or poly I:C

被引：29

作者：

Congleton, J ^{[1
]}

Sun, BL ^{[1
]}

机构：

[1] UNIV IDAHO, DEPT FISH & WILDLIFE RESOURCES, MOSCOW, ID 83843 USA

来源：

DISEASES OF AQUATIC ORGANISMS | 1996年 / 25卷 / 03期

关键词：

interferon; IHN virus; rainbow trout; macrophage; leucocyte; cytokine;

D O I：

10.3354/dao025185

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Leucocytes from the anterior kidneys of rainbow trout Oncorhynchus mykiss produced an interferon-like cytokine (ILC) or cytokines when stimulated with free infectious hematopoietic necrosis (IHN) virus, with cell membrane-associated (CMA) IHN virus on glutaraldehyde-fixed chinook salmon embryo cells, or with the synthetic polyribonucleotide Poly I:C. Peak yields of ILC were similar in leucocyte cultures stimulated by free virus or by CMA virus at optimal multiplicities of infection. The antiviral activity had both acid-resistant and acid-labile components, indicating production of several cytokines. Moreover, activity produced by free virus-stimulated leucocytes was significantly (p less than or equal to 0.05) more resistant to treatment at pH 2 than was activity produced by CMA virus-stimulated leucocytes. Leucocyte populations enriched for macrophages (but not for neutrophils or lymphocytes) demonstrated a significantly enhanced capacity for ILC production after stimulation by Poly I:C or free IHN virus. The capacity of macrophages for ILC production may be an important element of the nonspecific resistance of salmonids to IHN virus.

引用

页码：185 / 195

页数：11

共 41 条

[1] PLAQUING PROCEDURE FOR INFECTIOUS HEMATOPOIETIC NECROSIS VIRUS [J].

BURKE, JA ;

MULCAHY, D .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1980, 39 (04) :872-876

[2]

CAPOBIANCHI MR, 1985, P SOC EXP BIOL MED, V178, P551

[3] INVOLVEMENT OF RAINBOW-TROUT LEUKOCYTES IN THE PATHOGENESIS OF INFECTIOUS HEMATOPOIETIC NECROSIS [J].

CHILMONCZYK, S ;

WINTON, JR .

DISEASES OF AQUATIC ORGANISMS, 1994, 19 (02) :89-94

[4]

COHEN S, 1986, METHOD ENZYMOL, V119, P136

[5] ISOLATION OF LEUKOCYTES FROM THE ANTERIOR KIDNEY AND SPLEEN OF RAINBOW-TROUT IN A SELF-GENERATING DENSITY GRADIENT [J].

CONGLETON, JL ;

GREENLEE, AR ;

RISTOW, SS .

JOURNAL OF FISH BIOLOGY, 1990, 36 (04) :575-585

[6]

de Kinkelin P, 1974, C R Acad Hebd Seances Acad Sci D, V279, P445

[7]

De Maeyer E., 1988, INTERFERONS OTHER RE

[8] ANTIVIRAL ACTIVITY IN 4 SPECIES OF SALMONIDS FOLLOWING EXPOSURE TO POLY INOSINIC - CYTIDYLIC ACID [J].

EATON, WD .

DISEASES OF AQUATIC ORGANISMS, 1990, 9 (03) :193-198

[9] SOME PROPERTIES OF THE EPITHELIOMA-PAPULOSUM-CYPRINI (EPC) CELL-LINE FROM CARP CYPRINUS-CARPIO [J].

FIJAN, N ;

SULIMANOVIC, D ;

BEARZOTTI, M ;

MUZINIC, D ;

ZWILLENBERG, LO ;

CHILMONCZYK, S ;

VAUTHEROT, JF ;

DEKINKELIN, P .

ANNALES DE VIROLOGIE, 1983, 134 (02) :207-220

[10]

Finter N B, 1981, Methods Enzymol, V78, P14

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