Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants

被引：625

作者：

Traver, D

Paw, BH

Poss, KD

Penberthy, WT

Lin, S

Zon, LI

机构：

[1] Childrens Hosp Boston, Boston, MA 02115 USA

[2] Howard Hughes Med Inst, Boston, MA 02115 USA

[3] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA

[4] Univ Calif Los Angeles, Los Angeles, CA 90095 USA

来源：

NATURE IMMUNOLOGY | 2003年 / 4卷 / 12期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/ni1007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The zebrafish is firmly established as a genetic model for the study of vertebrate blood development. Here we have characterized the blood-forming system of adult zebrafish. Each major blood lineage can be isolated by flow cytometry, and with these lineal profiles, defects in zebrafish blood mutants can be quantified. We developed hematopoietic cell transplantation to study cell autonomy of mutant gene function and to establish a hematopoietic stem cell assay. Hematopoietic cell transplantation can rescue multilineage hematopoiesis in embryonic lethal gata1(-/-) mutants for over 6 months. Direct visualization of fluorescent donor cells in embryonic recipients allows engraftment and homing events to be imaged in real time. These results provide a cellular context in which to study the genetics of hematopoiesis.

引用

收藏

页码：1238 / 1246

页数：9

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