Isolation of microsatellites from two species of scleractinian coral

被引:44
作者
Miller, KJ [1 ]
Howard, CG [1 ]
机构
[1] Univ Wollongong, Dept Biol Sci, Inst Conservat Biol, Wollongong, NSW 2522, Australia
来源
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 01期
关键词
Cnidaria; coral; Goniastrea favulus; microsatellites; Platygyra daedalea; population genetics;
D O I
10.1046/j.1471-8286.2003.00555.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microsatellites were isolated from two broadcast-spawning species of scleractinian coral (Platygyra daedalea and Goniastrea favulus) from Australia's Great Barrier Reef. We found 27 microsatellites across both species, although only five loci were polymorphic in each species. Microsatellite loci displayed a wide range of diversity levels with four to 11 alleles per locus (H-O = 0.26-0.91) in P. daedalea and two to seven alleles per locus (H-O = 0.16-0.96) in G. favulus. Most loci showed departures from Hardy-Weinberg equilibrium which may reflect nonrandom mating but may also be related to difficulties associated with coral DNA.
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页码:11 / 13
页数:3
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